Selection marker
AssigneeSyngenta Mogen B.V.
InventorBrigitte Damm
Life of this patent
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Abstract
This invention is concerned with the use of cyanamide hydratase as a selection marker in plant transformation. Cyanamide acts as a herbicide and plants transformed with the gene coding for cyanamide hydratase are able to convert the cyanamide into urea which enables the selection of transformed plants by survival under cyanamide pressure.
Claims19
A method for selecting transformed monocotyledonous plant cells, plant tissue, or plant parts, the method comprising:
- (a) obtaining a vector comprising a coding sequence for cyanamide hydratase and a coding sequence for a gene of interest, said coding sequence for cyanamide hydratase comprising SEQ ID NO:1;
- (b) transforming monocotyledonous plant cells with said vector;
- and
- (c) selecting monocotyledonous plant cells, plant tissues, or plant parts comprising transformed monoctyledonous plant cells that are tolerant to cyanamide in a tissue culture medium comprising cyanamide.
A method for selecting tranformed plant cells, comprising:
- (a) tranforming plant cells with a vector comprising a nucleic acid sequence coding for cyanamide hydratase, said nucleic acid sequence comprising SEQ ID NO:1;
- (b) growing said plant cells or progeny of said plant cells in a tissue culture medium comprising cyanamide;
- and
- (c) selecting transformed plant cells which survive exposure to said cyanamide.
A method for selecting transformed monocotyledonous plant cells, plant tissue, or plant parts, the method comprising:
- (a) growing monocotyledonous plant cells, plant tissue, or plant parts comprising a nucleic acid sequence of SEQ ID NO:1 in a medium comprising cyanamide;
- and
- (b) selecting transformed plant cells, plant tissue, or plant parts which survive exposure to said cyanamide.
A transgenic monocotyledonous plant comprising a nucleotide sequence coding for cyanamide hydratase and a nucleotide sequence coding for a gene of interest, wherein said nucleotide sequence coding for cyanamide hydratase is used as a selectable marker and comprises SEQ ID NO:1.
A method for selecting transformed dicotyledonous plant cells, plant tissue, or plant parts, the method comprising:
- (a) obtaining a vector comprising a coding squence for cyanamide hydratase and a coding sequence for a gene or interest;
- (b) transforming dicotyledonous plant cells with said vector;
- and
- (c) selecting dicotyledonous plant cells, plant tissue, or plant parts comprising transformed dicotyledonous plant cells that are tolerant to cyanamide in a tissue culture medium comprising cyanamide;
- wherein said coding sequence for cyanamide hydratase is used as a selectable marker and comprises SEQ ID NO:1.
A method for selecting transformed dicotyledonous plant cells, plant tissue, or plant parts, the method comprising:
- (a) growing dicotyledonous plant cells, plant tissue, or plant parts transformed with a nucleic acid sequence comprising SEQ ID NO:1 in a medium comprising cyanamide;
- and
- (b) selecting transformed plant cells, plant tissue, or plant parts which survive exposure to said cyanamide.
A transgenic dicotyledonous plant comprising a nucleotide sequence coding for cyanamide hydratase and a nucleotide sequence coding for a gene of interest, wherein said nucleotide sequence coding for cyanamide hydratase is used as a selectable marker and comprises SEQ ID NO:1.
Dependencies are inferred from the claim text.