US 6,660,908 B2 · Genetic sequences encoding substrate-specific dihydroflavonol 4-reductase and uses therefor

Abstract

The invention includes modified dihydroflavonol 4-reductase (DFR) nucleic acids encoding the modified DFR that has altered amino acid sequences at the substrate specificity determining region. The property of the modified DFR is characterized by its ability to reduce dihydrokaempferol (DHK) preferentially over dihydroquercetin (DHQ), and dihydromyricetin (DHM). The invention also includes plants having at least one cell expressing the modified DFR. Such plants are characterized by the increased content of pelargonidin-based pigments. The invention also includes vectors comprising at least a portion of the modified DFR nucleic acids. The invention also includes methods using such vectors for producing plants having the increased content of pelargonidin-based pigments.

Claims5

Dependent claims

A method for production a plant having a phenotype characterized by an increased production of leucopelap-gonidin compared to wild type plants comprising the steps of:

(i) isolating a first nucleic acid comprising SEQ ID NO:
1 encoding a dihydroflavanol-4-reductase;
(ii) carrying out site specific mutagenesis on said first nucleic acid to produce a second nucleic acid wherein the codon for the Asn residue at position 134 has been mutated to a codon for Leu;
(iii) introducing said second nucleic acid into a vector wherein said second nucleic acid is operable linked to a promoter
(iv) transforming a plant cell with said vector;
(v) regenerating the plants from one or more of said transformed plant cells;
(vi) selecting a plant with the desired phenotype.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/060,506Filed 30 Jan 2002 · division

Priority

14 Aug 2000

Published

US 2002/0120959 A129 Aug 2002

Granted

9 Dec 2003

Term ends

Not estimatedThe source records don’t support an estimate.

Classification4 codes

C12N 9/0071
Oxidoreductases: acting on paired donors with incorporation of molecular oxygen
C12N 9/0006
Oxidoreductases: acting on CH-OH groups as donors
C12N 15/825
Involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine: involving pigment biosynthesis
C12N
Microorganisms or enzymes

Genetic sequences encoding substrate-specific dihydroflavonol 4-reductase and uses therefor

Life of this patent

Genetic sequences encoding substrate-specific dihydroflavonol 4-reductase and uses therefor

Life of this patent

Abstract

Claims5