Genes encoding SCIP-1 orthologs and methods of use
AssigneePioneer Hi-Bred International, Inc.
InventorsCarol A. Hendrick, Xu Hu, Guihua Lu
Life of this patent
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Abstract
Compositions and methods for enhancing the resistance of plants to pests and for altering the level of biotin in plants are provided. Nucleotide sequences isolated from soybean, rice, maize, and wheat are provided. The nucleotide sequences encode orthologs of sunflower SCIP-1. Also provided are the SCIP-1 proteins encoded by such nucleotide sequences. The methods involve transforming plants with the SCIP-1 nucleotide sequences to enhance the plant's resistance to plant pests or to alter the level of biotin in the plant. Transformed plants, plant cells, tissues, and seeds are also provided.
Claims19
An isolated nucleotide molecule comprising a nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 5:
- (b) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 6;
- (c) a nucleotide sequence encoding a polypeptide having SCIP-1 biological activity, wherein said nucleotide sequence has at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 5;
- and
- an antisense nucleotide sequence corresponding to the nucleotide sequence of (a), (b), or (c).
A transformed plant comprising in its genome at least one stably incorporated nucleotide construct comprising a nucleotide sequence operably linked to a promoter capable of driving gene expression in a plant cell, said nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 5;
- (b) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 6;
- (c) a nucleotide sequence encoding a polypeptide having SCIP-1 biological activity, wherein said nucleotide sequence has at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 5;
- and
- an antisense nucleotide sequence corresponding to the nucleotide sequence of (a), (b), or (c).
A transformed plant cell comprising in its genome at least one stably incorporated nucleotide construct comprising a nucleotide sequence operably linked to a promoter capable of driving gene expression in a plant cell, said nucleotide sequence selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 5;
- (b) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 6;
- (c) a nucleotide sequence encoding a polypeptide having SCIP-1 biological activity;
- wherein said nucleotide sequence has at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 5;
- an antisense nucleotide sequence corresponding to the nucleotide sequence of(a), (b), or (c).
A method for increasing the resistance of a plant to a pathogen comprising stably incorporating in the genome of said plant a nucleotide construct comprising a nucleotide sequence operably linked to a promoter capable of driving gene expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 5;
- (b) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 6;
- and
- (c) a nucleotide sequence encoding a polypeptide having SCIP-1 biological activity, wherein said nucleotide sequence has at least 95% sequence identity to the nucleotide sequence set forth in SEQ ID NO:
- 5;
- wherein the resistance of said pathogen is increased.
Dependencies are inferred from the claim text.