Probe for diagnosing infectious diseases
AssigneeFuso Pharmaceutical Industries, Ltd.
InventorsHiroyuki Keshi, Soji Eda, Hirotsugu Uehara, Hiroshi Ueyama, Akio Matsuhisa
Life of this patent
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Abstract
A probe derived from bacteria of pneumonia, containing fragments of DNA which streptococcus pneumoniae essentially possesses, and useful for detecting and identifying causative bacteria of pneumonia is obtained by completely digesting the DNA with a restriction endonuclease PstI, followed by cloning into a suitable vector.
Claims15
A purified nucleic acid probe for diagnosing infectious diseases caused by Streptococcus pneumoniae, the nucleic acid consisting of a nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 1, 2, 5, 6, and 7, and nucleotide sequences fully complementary thereto.
A purified nucleic acid comprising of the nucleotide sequence set forth in SEQ ID NO:
- 4, or a nucleotide sequence fully complementary thereto, wherein said nucleic acid hybridizes to genomic DNA of Streptococcus pneumoniae, but fails to hybridize to genomic DNA of Streptococcus agalactiae, Streptococcus anginosus, Streptococcus constellatus, Streptococcus equisimilis, Streptococcus faecium, Streptococcus faecalis, Streptococcus mitis, Streptococcus morbillorum, Streptococcus pyogenes, Streptococcus sanguis, and Streptococcus salivarius, under the following hybridization and washing conditions:
- hybridization overnight at 42° C. in a hybridization solution comprising 45% formamide and 5×SSC;
- and
- washing twice for 20 minutes at 55° C., in a washing solution comprising 0.1×SSC and 0.1% SDS.
A purified polynucleotide consisting of a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, and nucleotide sequences fully complementary thereto, wherein said polynucleotide hybridizes to genomic DNA of Streptococcus pneumoniae, but fails to hybridize to genomic DNA of Streptococcus agalactiae, Streptococcus anginosus, Streptococcus constellatus, Streptococcus equisimilis, Streptococcus faecium, Streptococcus faecalis, Streptococcus mitis, Streptococcus morbillorum, Streptococcus pyogenes, Streptococcus sanguis, and Streptococcus salivarius, under the following hybridization and washing conditions:
- hybridization overnight at 42° C. in a hybridization solution comprising 45% formamide and 5×SSC;
- and
- washing twice for 20 minutes at 55° C., in a washing solution comprising 0.1×SSC and 0.1% SDS.
A purified nucleic acid probe for diagnosing infectious diseases caused by Streptococcus pneumoniae, wherein the nucleotide sequence of said nucleic acid consists of a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, and SEQ ID NO:
- 7, and nucleotide sequences fully complementary thereto.
A vector containing a nucleic acid insert, wherein the nucleic acid insert consists of a nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 1, 2, 5, 6, and 7, and nucleotide sequences fully complementary thereto.
Dependencies are inferred from the claim text.