Vesicular amino acid transporter composition and method
AssigneesThe Regents of the University of CA, University of Utah Research Foundation
InventorsRobert H. Edwards, Richard J. Reimer, Steven L. McIntire, Erik M. Jorgensen, Kim Schuske
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Abstract
Disclosed are invertebrate and vertebrate synaptic vesicle amino acid transporter compositions which define a novel family of transporter proteins, recombinant vectors and cells comprising nucleic acid sequences encoding vertebrate synaptic vesicle amino acid transporter proteins and antibodies directed against such proteins. Also disclosed are methods for utilizing such compositions in screening assays and treatment regimens.
Claims13
An isolated DNA fragment that encodes an amino acid synaptic vesicle transporter protein that is capable of effecting the passage of GABA into a synaptic vesicle, and which is capable of hybridizing to the complement of the nucleic acid disclosed in SEQ ID NO:
- 3 under high stringency conditions as follows:
- hybridization at 65° C. in about 5×SSPE and washing at 65° C. in about 0.1×SSPE.
An isolated DNA fragment that encodes an amino acid synaptic vesicle transporter protein which contains at least 10 putative transmembrane domain regions, each of which has a sequence that is at least 90% identical to a transmembrane region having a sequence which is disclosed in SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, SEQ ID NO:
- 13, or SEQ ID NO:
- 14, respectively, and wherein said protein is capable of effecting the passage of GABA into a synaptic vesicle.
A recombinant expression vector, comprising
- a DNA fragment coding for an amino acid synaptic vesicle transporter protein containing an amino acid sequence which has at least 90% sequence identity to SEQ ID NO:
- 2,
- and wherein said protein is capable of effecting the passage of GABA into a synaptic vesicle, and
- operably linked to said fragment, a regulatory sequence capable of promoting expression of the protein in a selected host.
Dependencies are inferred from the claim text.