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US 6,660,519 B2Granted
High throughput method, apparatus and kit for plating microorganisms and cell cultures
AssigneeParadigm Genetics, Inc.
InventorCarol Hamilton
Life of this patent
Estimating the term…
Abstract
The present invention relates to a highly effective method, apparatus and kit for growing and plating out cultures of microorganisms or cell culture, especially derived from culture blocks containing multiple wells, such as a 96-well or 384-well plate or block.
Claims54
A method for plating microorganisms or cell culture, comprising:
- a) using an apparatus comprising:
- i) a tray having a bottom and sidewalls extending from said bottom;
- ii) a plurality of parallel dividers, each divider having a length and a height and being substantially uninterrupted along the entire length thereof;
- iii) means for maintaining said dividers substantially parallel within said tray, wherein said dividers are removable from said tray;
- iv) agar that fills said tray to a point less than the height of said dividers in said tray, wherein said agar has a surface for plating microorganisms or cell culture that is defined as a lane between two adjacent dividers or a lane between a divider and an adjacent sidewall;
- and
- v) beads that can fit between said substantially parallel dividers;
- and
- b) contacting said beads with a source of microorganisms or cell culture;
- and
- c) rolling said beads with said microorganisms or cell culture along said agar surface from one end of the tray to the opposite end to plate said microorganisms or cell culture.
8 more elements
An apparatus comprising:
- a) a tray having a bottom and sidewalls extending from said bottom;
- b) a plurality of parallel dividers, each divider having a length and a height and being substantially uninterrupted along the entire length thereof;
- c) means for maintaining said dividers substantially parallel within said tray, wherein said dividers are removable from said tray;
- and
- d) beads that can fit between said substantially parallel dividers from one end of the tray to the opposite end.
2 more elements
An apparatus comprising:
- a) a tray having a bottom and sidewalls extending from said bottom;
- b) a plurality of parallel dividers, each divider having a length and a height and being substantially uninterrupted along the entire length thereof;
- c) means for maintaining said dividers substantially parallel within said tray, wherein said dividers are removable from said tray;
- d) a cover for covering said tray and said dividers;
- and
- e) beads that can fit between said substantially parallel divider from one end of the tray to the opposite end.
3 more elements
An apparatus comprising:
- a) a tray having a bottom and sidewalls extending from said bottom;
- b) a plurality of parallel dividers, each divider having a length and a height and being substantially uninterrupted along the entire length thereof;
- c) means for maintaining said dividers substantially parallel within said tray, wherein said dividers are removable from said tray;
- d) beads that fit between said substantially parallel dividers from one end of the tray to the opposite end;
- and
- e) agar that fills said tray to a point less than the height of said dividers in said tray, wherein said agar has a surface for plating microorganisms or cell culture that is defined as a lane between two adjacent dividers or a lane between a divider and an adjacent sidewall.
3 more elements
An apparatus comprising:
- a) a tray having a bottom and sidewalls extending from said bottom;
- b) a plurality of parallel dividers, each divider having a length and a height and being substantially uninterrupted along the entire length thereof;
- c) means for maintaining said dividers substantially parallel within said tray, wherein said dividers are removable from said tray;
- d) a cover for covering said tray and said dividers;
- e) beads that can fit between said substantially parallel dividers from one end of the tray to the opposite end;
- and
- f) agar that fills said tray to a point less than the height of said dividers in said tray, wherein said agar has a surface for plating microorganisms or cell culture that is defined as a lane between two adjacent dividers or a lane between a divider and an adjacent sidewall.
4 more elements
A kit comprising in a single package:
- a) a tray having a bottom and sidewalls extending from said bottom;
- b) a plurality of parallel dividers, each divider having a length and a height and being substantially uninterrupted along the entire length thereof;
- c) means for maintaining said dividers substantially parallel within said tray, wherein said dividers are removable from said tray;
- and
- d) beads that can fit between said substantially parallel dividers from one end of the tray to the opposite end.
2 more elements
Dependencies are inferred from the claim text.