Human lysozyme gene, it's encoded polypeptide and the method of preparing them
AssigneeYu Long
InventorsLong Yu, Qiang Fu, Yong Zhao, Honglai Zhang, Anding Bi
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Abstract
The invention relates to LYC4, a novel member of the lysozyme gene family. The invention provides the cDNA sequence encoding the novel lysozyme, the polypeptide encoded by the sequence, as well as methods for producing said novel human lysozyme utilizing recombinant technology. The invention also provides the use of the novel human lysozyme.
Claims12
An isolated DNA molecule having a nucleotide sequence encoding a polypeptide having the amino acid sequence of amino acids 20-146 of SEQ ID NO:4.
An isolated lysozyme polypeptide having the amino acid sequence of amino acids 20-146 of SEQ ID NO:4.
A method for producing a LYC4 protein which comprises:
- (a) introducing an expression vector for producing a LYC4 protein, said vector comprising a nucleotide sequence encoding a polypeptide having the amino acid sequence of amino acids 20-146 of SEQ ID NO:4, wherein said nucleotide sequence is operably linked to at least one expression control sequence, into a host cell, thereby forming a recombinant host cell;
- (b) culturing the recombinant host cell of (a) under conditions suitable for expression of the DNA molecule encoding the polypeptide, such that LYC4 protein is produced;
- and
- (c) isolating the LYC4 protein so produced.
Dependencies are inferred from the claim text.