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US 6,660,501 B2Granted
Production of proteins by cell culture
AssigneeLonza Group, AG
InventorRaymond Paul Field
Life of this patent
Estimating the term…
Abstract
A process for the production of a protein by cell culture, where the cells that produce the protein are cultured in the presence of a chemical agent that enhances the production of the protein.
Claims39
A process for obtaining a protein by culture of hybridoma cells comprising the steps of:
- (a) culturing animal hybridoma cells, comprising cells which produce said protein, in continuous presence of an alkanoic acid or salt thereof, which enhances protein production, wherein said alkanoic acid or salt thereof is present at a concentration range of 0.1 mM to 200 mM, wherein production of said protein is enhanced but at which cell growth is maintained, compared to production of said protein in the absence of said alkanoic acid or salt thereof;
- (b) continuing the culture until said protein accumulates;
- and
- (c) isolating said protein.
1 more element
The process for obtaining a protein by culture of hybridoma cells comprising the steps of:
- maintaining animal hybridoma cells, which produce said protein in culture in the continuous presence of an alkanoic acid or salt thereof which enhances protein production, wherein said alkanoic acid or salt thereof is present at a concentration range of 0.1 mM to 200 mM, wherein production of said protein is enhanced but at which cell viability is not significantly reduced compared to production of said protein in the absence of said alkanoic acid or salt thereof.
Dependencies are inferred from the claim text.