Production of peptides in plants as viral coat protein fusions
AssigneeLarge Scale Biology Corporation
InventorsThomas H. Turpen, Stephen J. Reinl, Laurence K. Grill
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Abstract
The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have many uses. Such uses include use as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity, including immunity against parasitic infections.
Claims10
A method of producing a protein of interest, comprising the steps:
- (a) introducing a polynucleotide into a plant or a plant cell, wherein said polynucleotide encodes a fusion protein which comprises a plant viral coat protein from a single-stranded plus-sense RNA virus fused to a protein of interest comprising four or more amino acids, wherein said polynucleotide further comprises a promoter functional in plants 5′ to the fusion protein encoding region;
- and
- (b) expressing said fusion protein in said plant or said plant cell.
Dependencies are inferred from the claim text.