CARK protein and nucleic acid molecules and uses therefor
AssigneeMillennnium Pharmaceuticals, Inc.
InventorJeyaseelan Raju
Life of this patent
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Abstract
The invention provides isolated nucleic acids molecules, designated CARK nucleic acid molecules. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing CARK nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a CARK gene has been introduced or disrupted. The invention still further provides isolated CARK proteins, fusion proteins, antigenic peptides and anti-CARK antibodies. Diagnostic methods utilizing compositions of the invention are also provided.
Claims18
A method for identifying a compound which binds to a polypeptide comprising the amino acid sequence of SEQ ID NO:2, comprising:
- contacting the polypeptide, or a cell expressing the polypeptide with a test compound;
- and
- determining whether the polypeptide binds to the test compound, thereby identifying a compound which binds to a polypeptide comprising the amino acid sequence of SEQ ID NO:2.
A method for identifying a compound which binds to a polypeptide which is encoded by the nucleotide sequence contained in the plasmid deposited with ATCC as Accession Number PTA-1530, comprising:
- contacting the polypeptide, or a cell expressing the polypeptide with a test compound;
- and
- determining whether the polypeptide binds to the test compound, thereby identifying a compound which binds to said polypeptide.
A method for identifying a compound which binds to a cardiac-related ankyrin-repeat protein kinase polypeptide comprising an amino acid sequence encoded by a nucleic acid molecule which hybridizes to a complement of a nucleic acid molecule consisting of SEQ ID NO:1 or 3, in 6×SSC at 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 65° C., comprising:
- contacting the polypeptide, or a cell expressing the polypeptide with a test compound;
- and
- determining whether the polypeptide binds to the test compound, thereby identifying a compound which binds to said polypeptide.
A method for identifying a compound which binds to a cardiac-related ankyrin-repeat protein kinase polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 90% identical to the nucleotide sequence of SEQ ID NO:1 or 3, comprising:
- contacting the polypeptide, or a cell expressing the polypeptide with a test compound;
- and
- determining whether the polypeptide binds to the test compound, thereby identifying a compound which binds to said polypeptide.
A method for identifying a compound which binds to a cardiac-related ankyrin-repeat protein kinase polypeptide comprising an amino acid sequence which is at least 90% identical to the amino acid sequence of SEQ ID NO:2, comprising:
- contacting the polypeptide, or a cell expressing the polypeptide with a test compound;
- and
- determining whether the polypeptide binds to the test compound, thereby identifying a compound which binds to said polypeptide.
A method for identifying a compound which binds to a polypeptide comprising at least 25 consecutive amino acid residues of the amino acid sequence of SEQ ID NO:2, wherein said polypeptide has kinase activity, comprising:
- contacting the polypeptide, or a cell expressing the polypeptide with a test compound;
- and
- determining whether the polypeptide binds to the test compound, thereby identifying a compound which binds to said polypeptide.
A method for identifying a compound which binds to a polypeptide comprising amino acid residues 463-716 of SEQ ID NO:2, comprising:
- contacting the polypeptide, or a cell expressing the polypeptide with a test compound;
- and
- determining whether the polypeptide binds to the test compound, thereby identifying a compound which binds to said polypeptide.
Dependencies are inferred from the claim text.