ATP extraction method
AssigneeBecton, Dickinson and Company
InventorsLeanne M. Schrecengost, Jon C. Wannlund, Robert W. Rosenstein
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Abstract
A method for extracting ATP from a biological sample is disclosed. The method involves introducing a cationic extractant and an anionic substance and then extracting ATP. The method may be used to assay for the presence of ATP in a biological sample or to determine the amount of ATP extracted from a biological sample. The method is particularly useful in detecting contamination on surfaces and in food products. A reagent, a test device and a test kit that involve the use of the method to detect contamination are also disclosed.
Claims15
A method for assaying for a presence of ATP in a sample, comprising:
- a) introducing a cationic extractant and an anionic substance, wherein said anionic substance is selected from the group consisting of sulfate ion and sodium dodecylsulfate (SDS);
- b) extracting said ATP from said sample;
- c) permitting luciferin and magnesium to react with said extracted ATP to form an ATP-magnesium-luciferin complex;
- d) allowing the ATP-magnesium-luciferin complex to interact with luciferase, wherein light is produced;
- and
- e) measuring an intensity of said light, wherein a presence of light corresponds to a presence of said ATP.
A method for detecting an amount of ATP extracted from a sample, comprising:
- a) introducing a cationic extractant and an anionic substance, wherein said anionic substance is selected from the group consisting of sulfate ion and sodium dodecylsufate (SDS);
- b) extracting said ATP;
- c) permitting luciferin and magnesium to react with said extracted ATP to form an ATP-magnesium-luciferin complex;
- d) allowing the ATP-magnesium-luciferin complex to interact with luciferase, wherein light is produced;
- and
- e) measuring an intensity of said light, wherein said intensity of said light corresponds to an amount of ATP extracted.
A method for detecting contamination in a sample, comprising:
- a) introducing a cationic extractant and an anionic substance, wherein said anionic substance is selected from the group consisting of sulfate ion and sodium dodecylsulfate (SDS);
- b) extracting said ATP;
- c) permitting luciferin and magnesium to react with said extracted ATP to form an ATP-magnesium-luciferin complex;
- d) allowing the ATP-magnesium-luciferin complex to interact with luciferase, wherein light is produced;
- and
- e) measuring an intensity of said light, wherein said intensity of said light corresponds to a presence of said ATP, and wherein said presence of said ATP corresponds to contamination.
Dependencies are inferred from the claim text.