Antibodies to human goose-type lysozyme
AssigneeIncyte Corporation
InventorsPreeti Lal, Karl J. Guegler, Neil C. Corley, Chandra Patterson
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Abstract
The invention provides a human goose-type lysozyme (GOLY) and polynucleotides which identify and encode GOLY. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating or preventing disorders associated with expression of GOLY.
Claims13
An isolate antibody which specifically binds with high affinity to a polypeptide comprising an amino acid sequence of SEQ ID NO:1, wherein the antibody binds to an epitope of a polypeptide consisting of the sequence of SEQ:
- ID NO:
- 1.
A method preparing a polyclonal antibody, the method comprising:
- a) immunizing an animal with a polypeptide consisting of an amino acid sequence of SEQ ID NO:1 under conditions to elicit an antibody response,
- b) isolating antibodies from the animal, and
- c) screening the isolated antibodies with the polypeptide, thereby identifying a polyclonal antibody which specifically binds with high affinity to a polypeptide comprising an amino acid sequence of SEQ ID NO:1.
A method of making a monoclonal antibody, the method comprising:
- a) immunizing an animal with a polypeptide consisting of an amino acid sequence of SEQ ID NO:1 under conditions to elicit an antibody response,
- b) isolating antibody producing cells from the animal,
- c) fusing the antibody producing cells with immortalized cells to form monoclonal antibody-producing hybridoma cells,
- d) culturing the hybridoma cells, and
- e) isolating from the culture a monoclonal antibody which specifically binds with high affinity to a polypeptide comprising an amino acid sequence of SEQ ID NO:1.
Dependencies are inferred from the claim text.