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US 6,660,473 B1Granted
C-terminal protein tagging
AssigneePhylos, Inc.
InventorsPeter Lohse, Michael McPherson, Robert G. Kuimelis
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Abstract
In general, the invention features proteins having covalently bonded C-terminal puromycin tags and methods for their production.
Claims18
A method for C-terminal protein tagging, comprising
- (a) providing a nucleic acid sequence encoding a protein;
- (b) translating said nucleic acid sequence under conditions in which translation stalls at the 3′ end of said nucleic acid sequence, forming a stalled translation complex comprising said protein;
- and
- (c) contacting said stalled translation complex with a puromycin-tag under conditions in which said puromycin-tag is covalently bonded to the C-terminus of said protein.
1 more element
Dependencies are inferred from the claim text.