Lysis method for micro-organisms

Method of lysing a biological sample comprising at least one microorganism, in order to release at least one nucleic material of interest belonging to said microorganism, said microorganism being of a bacterium type or a yeast type, wherein:

• said biological sample in a liquid medium is placed in a container,
• at least one particulate material which is substantially inert relative to the nucleic material is placed in said container,
• the mixture of biological sample and particulate material is subjected to a movement,
• whereby, in combination:
• the particulate material consists of beads having a diameter ranging from 90 μm to 150 μm when said microorganism is of the bacterium type, and having a diameter of about 500 μm when said microorganism is of the yeast type, and
• the movement chosen is of the vortex type, and corresponds to the following conditions:
• the apparent volume of the beads, Vb, and the volume of the liquid sample, Ve, are linked by the relationship Ve=α×Vb, wherein Vb<Ve, with a between 1.4 and 10 when the container is in tubular form, and α less than or equal to 2.1 when the container is in disk form,
• whereby, without one or more additional operating steps, the nucleic material is released directly into the liquid medium in the native state and in a state accessible to any reagent for subsequent treatment.