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AssigneeThe Central Science Laboratory (CSL) representing the Secretary of State of Environment, Food and Rural Affairs
InventorMichael F Sanders
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A method for detection, identification and/or quanification of target organisms of specific bacterial genus, species or serotype, based upon the occurrence of release of cell contents, particularly nucleotides, e.g., ATP, on lysis of bacterial cell walls on incubation with bacteriophages (phages) specific for them. When new phase particles are released at the end of the phage replication cycle nucleotide levels are measured and compared with controls. The method provides for the detection of specific bacteria which does not require insertion of the lux gene into the phage genome yet is faster and more sensitive than known nonmodified phage utilizing techniques. The method is only limited by the availability of phage types suitable for selective attack of the target bacterial to be detected and can detect a single Salmonella in a sample of milk in under 12 hours.
A method for identifying a target bacteria of a specific bacterial genus, species or serotype present in a sample comprising the steps of:
A method for quantifying a target bacteria of a specific bacterial genus, species or serotype present in a sample comprising the steps of:
A method for identifying the presence of bacteria of a specific bacterial genus, species or serotype present in a sample comprising the steps of:
Dependencies are inferred from the claim text.