Functionalization of substrate surfaces with silane mixtures

A process for preparing a solid substrate of low surface energy and functionalized with support-bound cleavable ligands, comprising:

• (a) producing a solution of an organic solvent and water;
• (b) contacting a surface of the substrate, which surface has reactive moieties thereon, with the solution and a derivatizing composition comprising a first silane, R1—Si(RLRXRY) and a second silane, R2—(L)n—Si(RLRXRY), so that —Si—R1 groups and —Si—(L)n—R2 groups become covalently coupled to the surface, wherein the RL moieties, which may be identical or different, are leaving groups, RX and RY are independently lower alkyl or leaving groups, R1 is a chemically inert moiety that lowers the surface energy of the substrate, n is 0 or 1, L is a linking group, and R2 is either a functional group or a modifiable group that may be converted to such a functional group;
• if R2 is a modifiable group, converting it to a functional group;
• and
• (c) coupling a ligand to R2 through a linking moiety containing a chemically cleavable site selected from the group consisting of base-cleavable sites, acid-cleavable sites and nucleophile-cleavable sites.

A process for synthesizing an oligomer array on a solid substrate comprising:

• (a) producing a solution of an organic solvent and water;
• (b) contacting a surface of the substrate, which surface has reactive moieties thereon, with the solution and a derivatizing composition comprising a first silane, R1—Si(RLRXRY) and a second silane, R2—(L)n—Si(RLRXRY), so that —Si—R1 groups and —Si—(L)n—R2 groups become covalently coupled to the surface, wherein the RL moieties, which may be identical or different, are leaving groups, RX and RY are independently lower alkyl or leaving groups, R1 is a chemically inert moiety that lowers the surface energy of the substrate, n is 0 to 1, L is a linking group, and R2 is either a functional group enabling covalent binding of a molecular moiety or a modifiable group that may be converted to such a functional group;
• and
• (c) synthesizing a plurality of substrate-bound oligomers at each R2 by successively coupling monomers thereto, thereby forming an oligomer array on the functionalized surface provided in step (b).

A process for preparing an oligomer array, comprising:

• (a) producing a solution of an organic solvent and water;
• (b) contacting a surface of a substrate, which surface has reactive moieties thereon, with the solution and a derivatizing composition comprising a first silane, R1—Si(RLRXRY) and a second silane, R2—(L)n—Si(RLRXRY), so that —Si—R1 groups and —Si—(L)n—R2 groups become covalently coupled to the surface, wherein the RL moieties, which may be identical or different, are leaving groups, RX and RY are independently lower alkyl or leaving groups, R1 is a chemically inert moiety that lowers the surface energy of the substrate, n is 0 to 1, L is a linking group, and R2 is either a functional group enabling covalent binding of a molecular moiety or a modifiable group that may be converted to such a functional group;
• and
• (c) binding a plurality of substrate-bound oligomers to the functionalized substrate by coupling an oligomer to each R2.

A process for preparing a substrate of low surface energy and functionalized with support-bound cleavable ligands, comprising:

• (a) producing a solution of an organic solvent and water;
• (b) contacting a surface of the substrate, which surface has reactive moieties thereon, with the solution and a derivatizing composition comprising a first silane, R1—Si(RLRXRY) and a second silane, R2—(L)n—Si(RLRXRY), so that —Si—R1 groups and —Si—(L)n—R2 groups become covalently coupled to the surface, wherein the RL moieties, which may be identical or different, are leaving groups, RX and RY are independently lower alkyl or leaving groups, R1 is a chemically inert moiety that lowers the surface energy of the substrate, n is 0 to 1, L is a linking group, and R2 is either a functional group or a modifiable group that may be converted to such as functional group;
• if R2 is a modifiable group converting it to a functional group;
• and
• (c) coupling a ligand to R2 through a linking moiety containing a chemically cleavable site cleavable by an agent selected from the group consisting of lithium aluminum hydride, aluminum trichloride, silver ions, mercuric ions, and fluoride ions.