Bordetella strain expressing the FHA hybrid, liposomes and vaccines
AssigneeInstitut Pasteur de Lille
InventorsNathalie Mielcarek, Camille Locht, Gilles Riveau, Odile Poulain-Godefroy, André Capron
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Abstract
The invention concerns a Bordetella strain deficient in the production of toxin and expressing a hybrid protein comprising at least part of the filamentous hemagglutin (FHA) and at least part of a protein heterologous to FHA. The gene coding for the toxin has been eliminated, or at least partially deleted or mutated so as to produce an inactive toxin. This strain can be used as vaccine. The invention also concerns liposomes containing at least part of the FHA protein and at least one protein heterologous to the FHA protein, and the use of FHA for the stimulation of immune responses.
Claims8
Bordetella pertussis strain BPNX deposited under accession number I-1770 on Oct. 8, 1996, in the National Collection of Microorganism Cultures of “l'Institut Pasteur” (CNCM).
A pharmaceutical composition containing a pharmacologically efficient quantity of a Bordetella strain deficient in the production of pertussis toxin which, by elimination or deletion of the gene coding for the pertussis toxin, does not produce pertussis toxin,
- wherein said Bordetella strain expresses a hybrid protein comprising Bordetella pertussis filamentous hemagglutinin and a protein heterologous to said Bordetella pertussis filamentous hemagglutinin;
- wherein said deficient Bordetella strain induces a higher production of antibodies against said filamentous hemagglutinin than the wild-type parent Bordetella pertussis strain;
- and
- wherein said deficient Bordetella strain colonizes lungs of an animal to which said pharmaceutical composition is administered.
The pharmaceutical composition comprising a pharmacologically efficient quantity of Bordetella pertussis strain BPNX deposited under accession number I-1770 on Oct. 8, 1996, in the National Collection of Microorganism Cultures of “l'Institut Pasteur” (CNCM).
Dependencies are inferred from the claim text.