Bioprosthetic heart valves
AssigneeMayo Foundation for Medical Education and Research
InventorNalini M. Rajamannan
Life of this patent
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Abstract
The invention provides methods and materials related to heart valves and the treatment of valvular heart disease. Specifically, the invention provides non-murine heart valve cells and heart valve cusps as well as methods for making heart valves. The invention also provides methods and materials for (1) slowing heart valve degeneration, thrombosis, and calcification, (2) treating carcinoid heart disease, (3) identifying inhibitors of heart valve degeneration, thrombosis, and calcification, and (4) determining the safety of drugs.
Claims20
An isolated heart valve cusp, wherein a cell of said cusp contains an exogenous nucleic acid that encodes a nitric oxide synthase, and wherein said cell expresses said nitric oxide synthase and has a reduced susceptibility to calcification.
A method for making a bioprosthetic heart valve cusp, said method comprising:
- a) obtaining a heart valve cusp, and
- b) introducing nucleic acid into a cell of said cusp, wherein said nucleic acid encodes a nitric oxide synthase, and wherein said cell expresses said nitric oxide synthase and has a reduced susceptibility to calcification.
A method for providing a mammal with a non-murine heart valve, said method comprising:
- a) providing an isolated non-murine heart valve;
- b) introducing a nucleic acid encoding a nitric oxide synthase into a cell of said heart valve such that said cell expresses said nitric oxide synthase, and
- c) introducing said heart valve of step (b) into said mammal, wherein said heart valve is an autograft or allograft.
A method for providing a mammal with a non-murine heart valve, said method comprising:
- a) providing an isolated non-murine heart valve;
- b) introducing a nucleic acid encoding a nitric oxide synthase into a cell of said heart valve such that said cell expresses said nitric oxide synthase,
- c) fixing or freezing said heart valve of step (b), and
- d) introducing said heart valve of step (c) into said mammal, wherein said heart valve is a xenograft.
Dependencies are inferred from the claim text.