Electrophoretic method and electrophoretic instrument therefor
AssigneeHitachi, Ltd.
InventorsTomohiro Shoji, Yoshiyuki Okishima, Masaya Kojima, Muneo Maeshima
Life of this patent
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Abstract
An injection syringe 14 for injecting a gel into a capillary 111, a charging syringe 15 for charging the injection syringe 14 with the gel 10 are attached to a block 17. A check valve 16 is inserted between the both syringes, and the check valve 16 works so as to prevent the gel 10 from flowing back to the charging syringe 15. As the result, the gel charging can be automated, and accordingly the processing ability of the electrophoretic instrument can be improved.
Claims12
An electrophoresis method comprising the steps of supplying a gel for electrophoresis to a bore of an injection syringe;
- driving a plunger of said injection syringe by a motor;
- injecting the gel into a bore of a capillary through a block having a flow passage communicating to said capillary;
- and supplying a sample to be analyzed to said capillary, and separating said sample by electrophoresis, the method further comprising the steps of:
- detecting the amount of the gel remaining in said injection syringe;
- driving a plunger of a charging syringe to charge said flow passage in said block with the gel from the charging syringe when the value of the amount of gel detected is smaller than a predetermined amount of the gel that should be in the injection syringe, the charging syringe being connected to said flow passage in aid block through a check valve;
- then
- supplying the gel in said in injection syringe to said capillary by the plunger of said injection syringe.
An electrophoretic instrument comprising:
- a capillary;
- a buffer reservoir;
- a gel injection mechanism for injecting a gel of a sample separating medium into said capillary comprising:
- an injection syringe with a plunger for injecting gel into said capillary;
- a charging syringe with a plunger for performing charging with gel;
- a block having a flow passage that connects said injection syringe, said charging syringe, said capillary and said buffer reservoir;
- and
- a check valve for preventing gel from flowing back to said charging syringe, said check valve being arranged between said charging syringe and said flow passage;
- and
- an encoder for determining an amount of gel in said injection syringe;
- and
- a detecting unit for detecting a sample introduced into said capillary and electrophoretically separated.
An electrophoretic instrument comprising:
- a capillary;
- a buffer reservoir;
- a gel injection mechanism or injecting a gel of a sample separating medium into said capillary comprising:
- an injection syringe with a plunger for injecting gel into said capillary;
- a charging syringe with a plunger for performing charging with gel;
- a block having a flow passage that connects said injection syringes, said charging syringe, said capillary and said buffer reservoir;
- and
- a check valve for preventing gel from flowing back to said charging syringe, said check valve being arranged between said charging syringe and said flow passage;
- and
- an encoder for detecting positional information of a drive unit;
- and
- a detecting unit for detecting a sample introduced into said capillary and electrophoretically separated.
Dependencies are inferred from the claim text.