US 6,656,734 B1Granted

Compositions for the delivery of polynucleotides to cells

AssigneeTransgene S.A.

InventorsRainer Bischoff, Hanno Kolbe, Klaus Schughart

Life of this patent

Estimating the term…

Loading patent…

Abstract

The present invention relates to a composition which is intended for transferring at least one therapeutically active substance into the interior of a target cell, characterized in that it comprises a mixture of at least one therapeutically active substance and at least one polar compound which is selected from a specific group of aprotic polar compounds. The therapeutically active substance is preferably a polynucleotide, and the polar compound is DPSO, which improves the ability of the polynucleotide to be transfected into the interior of the cells. The compositions according to the invention can be used as a diagnostic therapeutic, prophylactic or vaccinal medicament for treating the human or animal body by means of gene therapy.

Claims18

Dependent claims

A process for transferring a therapeutically active polynucleotide into a target cell in vitro, wherein said cell is brought into contact with a composition comprising a mixture of at least one therapeutically active polynucleotide and at least one aprotic polar compound selected from the group consisting of:

(a) formula I:
wherein R1 and R2 are identical or different alkyl radicals of 1 to 8 carbon atoms which are optionally substituted,
with the proviso that when one of R1 or R2 is a radical of 1 or 2 carbon atoms, the other is a radical of at least 3 carbon atoms;
wherein the volume of the aprotic polar compound represents from 5 to 50% of the total volume of the composition,
and wherein the polynucleotide prior to being part of the mixture, is free of any compound which facilitates its introduction into a target cell.

2 more elements

Dependencies are inferred from the claim text.