Animal cells and processes for the replication of influenza viruses
AssigneeChiron Behring GmbH & Co.
InventorsAlbrecht Gröner, Jürgen Vorlop
Life of this patent
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Abstract
Animal cells are described which can be infected by influenza viruses and which are adapted to growth in suspension in serum-free medium. Processes for the replication of influenza viruses in cell culture using these cells are furthermore described, as well as vaccines which contain the influenza viruses obtainable by the process or constituents thereof
Claims43
An MDCK cell which can be infected by influenza viruses, wherein the MDCK cell is adapted to growth in suspension, and wherein the MDCK cell is adapted to growth in serum-free medium.
A process for the replication of influenza viruses in cell culture, which comprises:
- (i) proliferating MDCK cells wherein said cells can be infected by influenza viruses, wherein said cells are adapted to growth in suspension, and wherein said cells are adapted for growth in serum-free medium;
- (ii) infecting the cells with influenza viruses;
- (iii) shortly before infection, simultaneously to infection, or shortly after infection, adding to the cell suspension a protease to cleave the precursor protein of hemagglutinin;
- and
- (iv) isolating the influenza viruses replicated in the cells.
A process for the replication of influenza viruses in cell culture, which comprises:
- (i) proliferating MDCK cells wherein said cells can be infected by influenza viruses and wherein said cells are adapted for growth in suspension;
- (ii) infecting the cells with influenza viruses;
- (iii) shortly before infection, simultaneously to infection, or shortly after infection adding to the cell suspension a protease to cleave the precursor protein of hemagglutinin;
- and
- (iv) isolating the influenza viruses replicated in the cells.
Dependencies are inferred from the claim text.