12832, a novel human kinase-like molecule and uses thereof
AssigneeMillennium Pharmaceuticals, Inc.
InventorsRachel Meyers, Martin R. Hodge, Mark Williamson
Life of this patent
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Abstract
Novel human kinase-like polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length kinase-like proteins, the invention further provides isolated kinase-like fusion proteins, antigenic peptides, and anti-kinase-like antibodies. The invention also provides kinase-like nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a kinase-like gene has been introduced or disrupted. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided.
Claims10
A method for identifying a compound which modulates the activity of a polypeptide selected from the group consisting of:
- (a) a polypeptide comprising the amino acid sequence of SEQ ID NO:2;
- b) a polypeptide comprising a fragment of the amino acid sequence of SEQ ID NO:2, wherein the fragment has a kinase protein activity and comprises at least 75 contiguous amino acids of SEQ ID NO:2;
- c) a polypeptide encoded by the nucleic acid sequence of SEQ ID NO:1;
- and
- d) a variant of the polypeptide comprising the amino acid sequence of SEQ ID NO:2, said variant having a kinase protein activity, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to a nucleic acid molecule comprising SEQ ID NO:1, nucleotides 191-1156 of SEQ ID NO:1, or a complement thereof under stringent conditions, said stringent conditions comprising hybridization in 6×SSC at 42° C., followed by washing with 1×SSC at 55° C.;
- comprising
- e) combining the polypeptide or a cell expressing the polypeptide with a test compound;
- and
- f) determining the effect of the test compound on the activity of the polypeptide, to thereby identify a compound which modulates the activity of the polypeptide.
A method for identifying a compound which binds to a polypeptide selected from the group consisting of:
- (a) a polypeptide comprising the amino acid sequence of SEQ ID NO:2;
- b) a polypeptide comprising a fragment of the amino acid sequence of SEQ ID NO:2, wherein the fragment has a kinase protein activity and comprises at least 75 contiguous amino acids of SEQ ID NO:2;
- c) a polypeptide encoded by the nucleic acid sequence of SEQ ID NO:1;
- and
- d) a variant of the polypeptide comprising the amino acid sequence of SEQ ID NO:2, said variant having a kinase protein activity, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to a nucleic acid molecule comprising SEQ ID NO:1, nucleotides 191-1156 of SEQ ID NO:1, or a complement thereof under stringent conditions, said stringent conditions comprising hybridization in 6×SSC at 42° C., followed by washing with 1×SSC at 55° C.;
- comprising the steps of:
- e) combining the polypeptide, or a cell expressing the polypeptide with a test compound;
- and
- f) determining whether the polypeptide binds to the test compound.
Dependencies are inferred from the claim text.