TCF-1 nucleotide sequence variation
AssigneeRoche Molecular Systems, Inc.
InventorsAnn Bethea Begovich, Henry Anthony Erlich, Andrew Grupe, Janelle Annette Noble, Gary Allen Peltz, Rebecca Lynne Reynolds, Karen Myra Walker, Gabriele Zangenberg
Life of this patent
Estimating the term…
Abstract
Methods and reagents for determining sequence variants present at the TCF-1 locus, which facilitates identifying individuals at risk for Th1 diseases, such as type 1 diabetes or multiple sclerosis, or Th2 diseases, such as allergic asthma or atopy.
Claims11
A method for characterizing an individual as possessing a factor contributing to an increased risk of type 1 diabetes or multiple sclerosis comprising:
- (a) determining the genotype of said individual with respect to the nucleotide present at position 883 of the TCF-1 gene;
- (b) classifying said individual based on the result obtained from step (a), wherein the presence of an A allele indicates a factor contributing to an increased risk of type 1 diabetes or multiple sclerosis.
A method for determining the genotype of a sample comprising a nucleic acid with respect to the nucleotide present in a TCF-1 gene at position 883, comprising:
- (a) contacting the nucleic acid with an oligonucleotide probe exactly complementary to an A allele or a C allele in a region encompassing position 883 under conditions such that hybridization occurs if and only if the A allele or the C allele is present;
- and
- (b) detecting if hybridization occurs, wherein, hybridization to the A allele indicates that the genotype of the sample corresponds to the A allele and hybridization to the C allele indicates that the genotype of the sample corresponds to the C allele.
A method for determining the genotype of a sample comprising a nucleic acid with respect to the nucleotide present in a TCF-l gene at position 883, comprising:
- (a) contacting the nucleic acid with one or more allele-specific primers specific for an A allele or a C allele under amplification conditions such that amplification occurs using said allele-specific primer if and only if the A allele or the C allele is present;
- and
- (b) detecting if amplifications occurs, wherein, amplification of the A allele indicates that the genotype of the sample corresponds to the A allele and amplification of the C allele indicates that the genotype of the sample corresponds to the C allele.
A method for determining the presence of an A allele or a C allele of a TCF-1 gene in a sample comprising a nucleic acid, comprising:
- (a) contacting the nucleic acid with an oligonucleotide exactly complementary to the A allele or the C at position 883 under stringent hybridization conditions;
- and
- (b) detecting hybridization wherein, hybridization to the A allele indicates the presence of the A allele and hybridization to the C allele indicates the presence of the C allele.
A method for characterizing an individual as possessing a factor contributing to an increased likelihood of having an increased IgE response comprising:
- (a) determining the genotype of said individual with respect to the nucleotide present at position 883 of the TCF-1 gene;
- (b) classifying said individual based on the result obtained from step (a), wherein the presence of a C allele indicates a factor contributing to an increased likelihood of having an increased IgE response.
Dependencies are inferred from the claim text.