Heat shock protein-based vaccines and immunotherapies
AssigneeSloan-Kettering Institute for Cancer Research
InventorsJames E. Rothman, F. Ulrich Hartl, Mee H. Hoe, Alan Houghton, Yoshizumi Takeuchi, Mark Mayhew
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Abstract
The present invention relates to methods and compositions for inducing an immune response in a subject, wherein the subject is administered an effective amount of at least one heart shock protein in combination with one or more defined target antigens. These methods and compositions may be used in the treatment of infectious diseases and cancers.
Claims32
A method for inducing an immune response to an infectious agent comprising administering to a subject a complex of:
- (a) a hybrid antigen comprising an antigenic domain of the infectious agent and a binding domain that non-covalently binds to an hsp70 and comprises a heptameric region having the sequence motif HyXHyXHyXHy (SEQ ID NO:29) where Hy represents a hydrophobic amino acid and X is any amino acid;
- and
- (b) an hsp70;
- wherein the hsp70 is from the same species as the subject;
- wherein the binding domain and the antigenic domain are covalently bound in vitro;
- and
- wherein the hybrid antigen and the hsp70 are non-covalently bound.
A method for inducing an immune response to an infectious agent comprising administering to a subject a complex of:
- (a) a hybrid antigen comprising an antigenic domain of the infectious agent and a binding domain comprising a peptide that non-covalently binds to an hsp70;
- and
- (b) an hsp70;
- wherein the hsp70 is from the same species as the subject;
- wherein the binding domain and the antigenic domain are covalently bound in vitro;
- and
- wherein the hybrid antigen and the hsp70 are non-covalently bound.
A method for inducing an immune response to an infectious agent comprising administering to a subject a complex of an hsp 70 and a hybrid antigen, the hybrid antigen consisting essentially of an antigenic domain of an infectious agent and a binding domain that non-covalently binds to an hsp70, wherein the antigenic domain is from a first source and the binding domain is from a second source different from the first source, and wherein the binding domain and the antigenic domain are covalently bound in vitro.
A method for inducing an immune response to an infectious agent comprising administering to a subject a complex of an hsp 70 and a hybrid antigen, the hybrid antigen consisting essentially of an antigenic domain of an infectious agent, a binding domain that non-covalently binds to an hsp70, and a peptide linker separating the antigenic domain and the binding domain, wherein the antigenic domain is from a first source and the binding domain is from a second source different from the first source, and wherein the binding domain, the peptide linker, and the antigenic domain are covalently bound in vitro.
Dependencies are inferred from the claim text.