43 Kd protein vaccine and method for the production thereof
AssigneeBoard of Trustees of Michigan State University
InventorsMartha H. Mulks, Brad J. Thacker, Maria Wilma T. Cruz
Life of this patent
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Abstract
A protein derivable from Actinobacillus pleuropneumoniae (APP) having a molecular size ranging between 43 Kd on SDS PAGE is described. The protein is soluble on sodium lauryl sarcosinate (SLS) and not heat modifiable. The protein can also be derived by recombinant methods. The protein in a vaccine provides immunity against multiple serotypes of APP.
Claims6
A vaccine for providing antibodies in swine against Actinobacillus pleuropneumoniae (APP) infection which consists essentially of:
- (a) a protein consisting essentially of an outer membrane protein of APP having a molecular size of 43 Kd as measured in SDS-PAGE, not heat modifiable and soluble in a 1% by weight to volume aqueous solution of sodium lauryl sarcosinate (SLS), wherein the protein is free of other naturally occurring proteins and antigens and of the APP as intact cells;
- and
- (b) a carrier.
A method for vaccinating a swine to provide antibodies against Actinobacillus pleuropneumoniae (APP) which consists essentially of:
- injecting the swine with a vaccine which contains a protein consisting essentially of an outer membrane protein (OMP) of the APP having a molecular size of 43 Kd as measured in SDS PAGE, not heat modifiable and soluble in sodium lauryl sarcosinate (SLS), wherein the outer membrane protein is free of other proteins and antigens and of the APP as intact cells.
Dependencies are inferred from the claim text.