Methods of screening agents for activity using teleosts
AssigneePhylonix Pharmaceuticals, Inc.
InventorsGeorge Serbedzija, Wen Lin Seng, Patricia McGrath
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Abstract
The present invention provides methods of screening an agent for activity using teleosts. Methods of screening an agent for angiogenesis activity, toxic activity and an effect cell death activity in teleosts are provided. The invention further provides high throughput methods of screening agents in multi-well plates.
Claims31
A method of screening an agent for a pharmacological activity, comprising:
- (a) providing a multi-well plate, the wells contaning teleosts in a medium and having a volume of 300 microliters or smaller per well;
- (b) introducing agents into different wells of the multi-well plate, and incubating the agents with the telcosts for sufficient time to induce a response in the teleosts indicative of the pharmacological activity;
- (c) introducing a labelling reagent into each well, which, through processing by or binding to a component of the teleost, generates a detectable signal dependent on the extent of the response in the teleost;
- (d) detecting the signal in each well to which an agent was introduced relative to the response from a control well lacking the agent as an indication of the pharmacological activity of the agent introduced in the well.
A method of monitoring distribution of an agent between teleosts and a surrounding medium:
- (a) providing a multi-well plate, the wells containing teleosts in a medium and having a volume of 300 microliters or smaller per well, and either he medium or the toleosts or both containing an agent;
- culturing the teleosts in the wells;
- (c) in each wall determining the amount of he agent in the medium, in the zebrafish or bot.
A method of screening an agent for a property comprising:
- (a) providing a multi-well plate, the wells containg teleosts in a medium and having a volume of 300 microliters or smaller per well;
- (b) introducing agents into different wells of the multi-well plate, and incubating the agents with the teleosts for sufficient time to induce a response;
- (c) detecting the response in each well to which an agent was introduced relative to the response from a control well lacking the agent as an indication of the property of the agent introduced in the well.
A method of screening an agent for a pharmacological activity, comprising:
- (a) providing a multi-well plate, the wells containing teleosts in a volume of 50-200 microliters per well;
- (b) introducing agents into different wells of the multi-well plate, and incubating the agents with the teleosts for sufficient time to induce a response in the teleosts indicative of the pharmacological activity;
- (c) introducing a labelling reagent into each well, which, through processing by or binding to a component of the teleost, generates a detectable signal dependent on the extent of the response in the teleost;
- (d) detecing the signal in each well to which an agent was intoduced relative to the response from a control well lacking the agent as an indication of the phamacological activity of the agent introduced in the well.
Dependencies are inferred from the claim text.