Skin and muscle-targeted gene therapy by pulsed electrical field
AssigneeGenetronics, Inc.
InventorsNagendu B. Dev, Gunter A. Hofmann, Edward Nolan, Dietmar P. Rabussay, Arnt Tonnessen, Georg Widera, Lei Zhang
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Abstract
The present invention describes an in vivo method, using pulsed electric field to deliver therapeutic agents into cells of the skin and muscle for local and systemic treatments. In particular, therapeutic agents include naked or formulated nucleic acid, polypeptides and chemotherapeutic agents.
Claims7
A micropatch electrode for use with an electroporation apparatus, said micropatch electrode comprising a substantially planar array of patch elements, each patch element comprising two sets of electrodes, wherein each set of electrodes comprises a first electrode and a second electrode electrically insulated from one another, such that when different electric potentials are applied to said first and second electrodes, a voltage is produced therebetween;
- wherein the first electrode and second electrode and the distance therebetween are sized to create an electroporation-causing electric field in skin or muscle when a voltage of at least 10 V/cm is applied thereto.
An electrode for use with an electroporation apparatus, said electrode comprising:
- a) a ring-shaped electrode having an electrically insulating shield with a centrally located through hole therein, wherein said electrically insulating shield provides support to said ring-shaped electrode and electrically insulates a tissue under treatment employing said electrode, and
- b) an electrically conducting injection needle for insertion through said through hole, said injection needle optionally comprising one or more injection holes disposed along its length and proximal to the needle tip, wherein said injection holes are in fluid communication with the hollow interior of said injection needle, and
- wherein the electrode, the injection needle and distance therebetween is sized to generate an electroporation-causing electric field when a potential difference of at least 10 V per cm of the distance is applied between said ring-shaped electrode and said needle electrode.
An electrode for use with an electroporation apparatus, said electrode comprising a suction generating device comprising a ring electrode disposed about an injection needle electrode, such that when said ring electrode is contacted with skin of a subject and suction is generated by said suction generating device, said skin is pulled up around the injection needle electrode, causing the injection needle electrode to pierce the skin, and
- wherein a potential difference applied to said ring-shaped electrode and said injection needle electrode creates a voltage therebetween.
Dependencies are inferred from the claim text.