Chromatography system for automatically separating different compounds in a sample

A chromatography system for automatically separating different compounds in a sample by chromatography, and collecting fractions of said different compounds of said sample in separate fraction wells, said system comprising

• a chromatography column connected to receive a sample and separate different compounds in said sample,
• a detector connected to receive said sample from said column and outputting a signal indicating the presence of compounds in said sample passing through said detector,
• a fraction collector connected to receive said sample from said detector for directing fractions of said sample to separate fraction wells, and
• an electronic controller receiving inputs from said detector and controlling said fraction collector,
• said electronic controller monitoring said signal from said detector over time and initiating a cut from one well to another at said fraction collector when said signal over time experiences a shoulder where the slope of said signal over time does not change in sign but does change by more than a predetermined amount, wherein said electronic controller determines to initiate a cut from one well to another when said slope is positive, and the slope at one point in time is less than the slope threshold, and the slope at a later time is greater than X times the slope threshold, where X is greater than one.

A chromatography system for automatically separating different compounds in a sample by chromatography, and collecting fractions of said different compounds of said sample in separate fraction wells, said system comprising

• a chromatography column connected to receive a sample and separate different compounds in said sample,
• a flow-through detector connected to receive said sample from said column and outputting a signal indicating the presence of compounds in said sample passing through said detector,
• a fraction collector connected to receive said sample from said detector for directing fractions of said sample to separate fraction wells, and
• an electronic controller receiving inputs from said detector and controlling said fraction collector,
• said electronic controller monitoring said signal from said detector over time and initiating a cut from one well to another at said fraction collector when said signal is less than a slope detection disable threshold and said signal over time experiences a specified change in slope condition.