Cytochrome P450 monooxygenases
AssigneesSyngenta Participations AG, Royal Veterinary Agricultural University
InventorsBarbara Ann Halkier, Sören Bak, Rachel Alice Kahn, Birger Lindberg Möller
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Abstract
Cytochrome P450II dependent monooxygenases and DNA molecules encoding these monooxygenases are provided, which are able to catalyze the biosynthethic conversion of aldoximes to nitrils and the conversion of said nitrils to the corresponding cyanohydrins, which are the presursors of cyanogenic glycosides. Moreover, the invention provides methods for obtaining DNA molecules according to the invention and methods for obtaining transgenic plants resistant to insects, acarids, or nematodes or plants with improved nutritive value.
Claims9
A cytochrome P450 monooxygenase that catalyzes the conversion of an aldoxime to the corresponding nitrile and the conversion of said nitrile to the corresponding cyanohydrin, wherein said cytochrome P450 monooxygenase comprises an amino acid sequence encoded by a nucleotide sequence, the complement of which hybridizes to SEQ ID NO:
- 1 under hybridization conditions comprising:
- hybridization in 5×SSC, 0.1% N-lauroylsarcosine, 0.02% SDS at 68° C., and washing twice for 15 minutes in 0.1×SSC, 0.1% SDS at 65° C.
Dependencies are inferred from the claim text.