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US 6,649,812 B1Granted
Method of production of transgenic plants, wholly performed in the To generation, from meristems
AssigneeBiogemma
InventorsNathalie Knittel, Philippe Lenee
Life of this patent
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Abstract
Method of production of transgenic plants wholly transformed into To generation. The method consists of a) genetically transforming a meristem explant; b) selective culturing for the specific development, among all the transformed cells, of those cells giving rise to secondary meristems and/or those cells capable of resulting in neoformed foliar meristems, c) regenerating, from the cellular material obtained during step b), transgenic plants.
Claims14
A method for producing a transgenic sunflower, said method comprising:
- a) genetically transforming a meristematic explant from said sunflower;
- b) culturing said transformed meristematic explant on a selection medium;
- c) detecting among axillary buds and/or newly formed leaf buds, the buds which are transformed;
- d) removing said transformed buds detected in step c) from the cultured transformed meristematic explant;
- e) culturing said transformed buds removed in step d) on a selection medium;
- f) repeating steps c), d) and e) at least three times;
- and,
- g) regenerating said cultured transformed buds to obtain a transgenic sunflower, wherein at least 92% of transgenic sunflowers are wholly transformed in the T0 generation.
5 more elements
Dependencies are inferred from the claim text.