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US 6,649,598 B2Granted
4,7-dichlorofluorescein dyes as molecular probes
AssigneeApplera Corporation
InventorsSteven M. Menchen, Linda G. Lee, Charles R. Connell, N. Davis Hershey, Vergine Chakerian, Sam L. Woo, Steven Fung
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Abstract
Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting specially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2′,4′,5′,7′-tetrachloro-4,7-dichloro-5-(and 6-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.
Claims36
A polynucleotide comprising a 4,7-dichlorofluorescein dye defined by the formula:
- wherein A′ is a linking group through which the dye is attached to said polynucleotide,
- B′ is carboxyl,
- X′ is hydrogen, and
- Z3 and Z4 are both hydrogen.
1 more element
A polynucleotide comprising a 4,7-dichlorofluorescein dye defined by the formula:
- wherein A′ is a linking group through which the dye is attached to said polynucleotide,
- B′ is carboxyl,
- X′ is hydrogen, and
- Z3 and Z4 are both chloro.
1 more element
Dependencies are inferred from the claim text.