Microparticles with multiple fluorescent signals and methods of using same
AssigneeLuminex Corporation
InventorsMark B. Chandler, Don J. Chandler
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Abstract
This invention provides a novel fluorescent particle including a core or carrier particle having on its surface a plurality of smaller polymeric particles or nanoparticles, which are stained with different fluorescent dyes. When excited by a light source they are capable of giving off multiple fluorescent emissions simultaneously, which is useful for multiplexed analysis of a plurality of analytes in a sample. The coupled complex particles carrying on their surface fluorescent nanoparticles, methods of preparing such polymer particles, and various applications and methods of using such particles are claimed.
Claims38
A carrier particle comprising a polymeric microparticle and at least one nanoparticle of at least one nanoparticle population coupled with said polymeic microparticle, wherein said at least one nanoparticle has a diameter in the range of 10-1,000 nm.
A carrier particle comprising a polymeric microparticle wherein said polymeric microparticle includes a plurality of nanoparticles of at least one nanoparticle population coupled with said polymeric microparticle.
A carrier particle comprising a microparticle and at least one nanoparticle of at least one nanoparticle population coupled with said microparticle wherein said nanoparticles in said at least one nanoparticle population are dyed with the same concentration of a dye.
A carrier particle comprising a microparticle and at least one nanoparticle of at least one nanoparticle population coupled with said microparticle wherein said microparticle is stained with a dye.
A plurality of carrier particles which include microparticles having nanoparticles of more than one nanoparticle population coupled with said microparticles.
A method of determining concentration of an analyte in a sample comprising the steps of:
- (a) providing a population of carrier particles which includes microparticles coupled with a subpopulation of nanoparticles, thereby providing a subpopulation of carrier particles, the subpopulation of nanoparticles being labeled so as to provide a specific subpopulation characteristic detectable by an instrument, the carrier particles of the specific carrier particle subpopulation having attached thereto a reagent which reacts with the analyte to form a bimolecular product,
- (b) contacting the sample with the population of carrier particles,
- (c) identifying the subpopulation of the carrier particles using the specific subpopulation characteristic, and
- (d) calculating the concentration of the analyte in the sample according to the bimolecular product detected for the subpopulation of carrier particles.
A method of determining concentration of a multiplicity of specific analytes in a sample comprising the steps of:
- (a) providing a population of carrier particles which includes microparticles coupled with a multiplicity of subpopulations of nanoparticles, thereby providing a multiplicity of subpopulations of carrier particles, each subpopulation of nanoparticles being labeled so as to provide a specific subpopulation characteristic detectable by an instrument, the carrier particles of a specific carrier particle subpopulation having attached thereto a specific reagent which reacts with a specific analyte to form a specific bimolecular product,
- (b) contacting the sample containing the multiplicity of specific analytes with the population of carrier particles,
- (c) identifying the multiplicity of subpopulations of the carrier particles using the specific subpopulation characteristics, and
- (d) calculating the concentrations of the specific analytes in the sample according to the specific bimolecular product detected for each subpopulation of carrier particle.
A kit for the detection of an analyte of interest, the kit comprising at least one polymeric microparticle with attached nanoparticles and at least one analytical reagent.
Dependencies are inferred from the claim text.