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US 6,649,408 B2Granted
Microdroplet cell culture technique
AssigneeGeorge Mason University
InventorsCharles L. Bailey, Ken Alibek
Life of this patent
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Abstract
The present invention comprises a novel culture method and device in which living cells are cultured in a plurality of individual microdroplets that are immobilized and isolated within a matrix of hydrophobic particles. The hydrophobic particles adhere to inoculated microdroplets of media, isolating the microdroplets in an aseptic microenvironmet. The plurality of individual microdroplets provide and optimal environment for the concentrated growth of cultured cells contained therein.
Claims12
A cell culture method comprising the steps of:
- introducing liquid media inoculated with cells to be cultured into a vessel;
- converting the inoculated liquid media into individual microdroplets;
- introducing a sufficient quantity of hydrophobic particles in the form of a dry powder into the vessel to coat the individual microdroplets;
- and
- growing the cells within the individual microdroplets.
2 more elements
Dependencies are inferred from the claim text.