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US 6,649,394 B1Granted
Topoisomerase IV, corresponding nucleotide sequences and uses thereof
AssigneeAventis Pharma S.A.
InventorsFrancis Blanche, Béatrice Cameron, Joël Crouzet, Alain Famechon, Lucia Ferrero
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Abstract
A novel topoisomerase IV, nucleotide sequences coding for said enzyme, corresponding vectors, and the use of said enzyme for screening biologically active materials.
Claims8
An isolated polypeptide resulting from the expression of a nucleotide sequence chosen from:
- (a) all or a part of the gr1A gene (SEQ ID NO:4) or all or a part of the gr1B gene (SEQ ID NO:6);
- or
- (b) sequences derived from the sequences of (a) because of the degeneracy of the genetic code,
- wherein the polypeptide expressed from all or a part of the gr1A gene exhibits topoisomerase activity in the presence of a Gr1B subunit and
- wherein the polypeptide expressed from all or a part of the gr1B gene exhibits topoisomerase activity in the presence of a Gr1A subunit.
2 more elements
An isolated polypeptide comprising SEQ ID NO:3, wherein the serine at amino acid position 80 has been changed to a tyrosine.
An isolated topoisomerase IV characterized in that it is capable of being obtained from the expression of a nucleotide sequence chosen from:
- (a) all or a part of the gr1A gene (SEQ ID NO:4) or all or a part of the gr1B gene (SEQ ID NO:6);
- or
- (b) sequences derived from the sequences of (a) because of the degeneracy of the genetic code,
- wherein the polypeptide expressed from all or a part of the gr1A gene exhibits topoisomerase activity in the presence of a Gr1B subunit and
- wherein the polypeptide expressed from all or a part of the gr1B gene exhibits topoisomerase activity in the presence of a Gr1A subunit.
2 more elements
Dependencies are inferred from the claim text.