DSP-11 dual-specificity phosphatase
AssigneeCEPTYR, Inc.
InventorsRalf M. Luche, Bo Wei
Life of this patent
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Abstract
Compositions and methods are provided for the treatment of conditions associated with cell proliferation, cell differentiation and cell survival. In particular, the dual-specificity phosphatase DSP-11, and polypeptide variants thereof that stimulate dephosphorylation of DSP-11 substrates, are provided. The polypeptides may be used, for example, to identify antibodies and other agents that inhibit DSP-11 activity. The polypeptides and agents may be used to modulate cell proliferation, differentiation and survival.
Claims12
An isolated polynucleotide that encodes a polypeptide comprising an amino acid sequence set forth in SEQ ID NO:2.
An isolated polynucleotide comprising the sequence set forth in SEQ ID NO:1.
An isolated polynucleotide that encodes a polypeptide capable of dephosphorylating an activated MAP-kinase, said polynucleotide comprising a sequence at least 80% identical to a polynucleotide that encodes a polypeptide comprising an amino acid sequence set forth in SEQ ID NO:2.
An isolated polynucleotide that encodes a polypeptide capable of dephosphorylating an activated MAP-kinase, said polynucleotide comprising a sequence at least 90% identical to a polynucleotide that encodes a polypeptide comprising an amino acid sequence set forth in SEQ ID NO:2.
An isolated polynucleotide that encodes a polypeptide capable of dephosphorylating an activated MAP-kinase, said polypeptide comprising an amino acid sequence of SEQ ID NO:2, wherein an aspartic acid is located at position 65 and the peptide sequence VGVHCALGFGRTGTMLACYLV (SEQ ID NO:3) is located at positions 91 through 111 of SEQ ID NO:2, wherein said polynucleotide comprises a sequence at least 80% identical to a polynucleotide that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2.
An isolated polynucleotide that encodes a polypeptide capable of dephosphorylating an activated MAP-kinase, said polypeptide comprising an amino acid sequence of SEQ ID NO:2, wherein aspartic acid is located at position 65 of SEQ ID NO:2 and the peptide sequence VGVHCALGFGRTGTMLACYLV (SEQ ID NO:3) is located at positions 91 through 111 of SEQ ID NO:2, wherein said polynucleotide comprises a sequence at least 90% identical to a polynucleotide that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2.
An isolated polynucleotide that detectably hybridizes to a polynucleotide having a sequence that is the complement of the sequence set forth in SEQ ID NO:1 under moderately stringent conditions that include a wash in 0.1×SSC and 0.1% SDS at 50° C. for 15 minutes, wherein said isolated polynucleotide exhibits at least 80% nucleotide identity to a polynucleotide comprising the sequence set forth in SEQ ID NO:1, and wherein said isolated polynucleotide encodes a polypeptide capable of dephosphorylating an activated Map-Kinase.
Dependencies are inferred from the claim text.