Thermophilic alkaline phosphoesterase and its expression
AssigneeFudan University
InventorsXiaoyu Sheng, Yumin Mao, Youzhong Yuan, Chaoneng Ji, Zongxiang Zhou
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Abstract
The invention belongs to the field of biological engineering techniques and relates to a thermophilic alkaline phosphoesterase. The invention provides an amino acid sequence of a thermophilic alkaline phosphoesterase of the invention, as well as a DNA fragment encoding the amino acid sequence. The present invention also includes methods for cloning an expression vector containing the DNA fragment or a portion thereof and for producing the recombinant enzyme. The invention also relates to a method for tagging biological macromolecules utilizing the enzyme.
Claims5
An isolated thermophilic alkaline phosphatase having the amino acid sequence of SEQ ID NO:2.
A recombinant and/or isolated thermophilic alkaline phosphoesterase comprising a polypeptide having the amino acid sequence of residues 1-475 of SEQ ID NO:2.
Dependencies are inferred from the claim text.