Methods of making polyhydroxyalkanoate using biosynthesis genes from alcaligenes latus
AssigneeLG Chemical Ltd.
InventorsJong-il Choi, Sang Yup Lee, Kyuboem Han
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Abstract
There are described methods for making polyhydroxyalkanoate and its copolymers, by culturing a host cell transformed with a vector comprising a polyhydroxyalkanoate biosynthesis-related DNA fragment isolated from Alcaligenes latus. The DNA fragment comprises genes that encode for polyhydroxyalkanoate synthase, β-ketothiolase, and acetoacetyl-CoA reductase.
Claims12
A method for producing polyhydroxyalkanoate (PHA) and its copolymers comprising culturing a host cell transformed with a vector comprising a PHA biosynthesis-related DNA fragment,
- wherein said DNA fragment is isolated from Alcaligenes latus and comprises three open reading frames operatively connected in the following order from 5′ to 3′:
- (a) a gene encoding PHA synthase whose protein sequence is set forth in SEQ ID NO:5,
- (b) a gene encoding β-ketothiolase whose protein sequence is set forth in SEQ ID NO:6, and
- (c) a gene encoding acetoacetyl-CoA reductase whose protein sequence is set forth in SEQ ID NO:7.
A method for producing polyhydroxyalkanoate and its copolymers, by culturing a host cell transformed with pKTC32.
A method for producing polyhydroxyalkanoate and its copolymers, by culturing an Alcaligenes eutropus deposited as KCTC 0568BP.
Dependencies are inferred from the claim text.