Multifunctional recombinant phycobiliprotein-based fluorescent constructs and phycobilisome display
AssigneeThe Regents of the University of California
InventorsAlexander N. Glazer, Yuping Cai
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Abstract
The invention provides multifunctional fusion constructs which are rapidly incorporated into a macromolecular structure such as a phycobilisome such that the fusion proteins are separated from one another and unable to self-associate. The invention provides methods and compositions for displaying a functional polypeptide domain on an oligomeric phycobiliprotein, including fusion proteins comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein. The fusion proteins provide novel specific labeling reagents.
Claims18
A fusion protein comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein.
A method for making a fusion protein, comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein, the method comprising the steps of:
- providing a nucleic acid encoding a polypeptide comprising a functional displayed domain and a functional phycobiliprotein domain;
- making the polypeptide by expressing the nucleic acid in a cell or cell-free expression system;
- and
- combining the polypeptide with a phycobiliprotein subunit under conditions to form the fusion protein.
Dependencies are inferred from the claim text.