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AssigneeMount Sinai School of Medicine of New York University
InventorsPeter Palese, Adolfo Garcia-Sastre, George G. Brownlee, Ervin Fodor
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The present invention relates methods of generating infectious negative-strand virus in host cells by an entirely vector-based system without the aid of a helper virus. In particular, the present invention relates methods of generating infectious recombinant negative-strand RNA viruses intracellularly in the absence of helper virus from expression vectors comprising cDNAs encoding the viral proteins necessary to form ribonucleoprotein complexes (RNPs) and expression vectors comprising cDNA for genomic viral RNA(s) (vRNAs) or the corresponding cRNA(s). The present invention also relates to methods of generating infectious recombinant negative-strand RNA viruses which have mutations in viral genes and/or which express, package and/or present peptides or polypeptides encoded by heterologous nucleic acid sequences. The present invention further relates the use of the recombinant negative-strand RNA viruses or chimeric negative-strand RNA viruses of the invention in vaccine formulations and pharmaceutical compositions.
A method for rescuing a chimeric recombinant negative strand RNA virus, wherein said chimeric virus expresses heterologous nucleic acid sequences, comprising:
A method for generating in cultured cells infectious viral particles of a chimeric negative strand RNA virus, wherein said chimeric virus expresses heterologous nucleic acid sequences and has greater than 3 genomic vRNA segments, comprising:
A method for generating in cultured cells infectious viral particles of a segmented chimeric negative-strand virus, wherein said chimeric virus expresses heterologous nucleic acid sequences, said method comprising:
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