Process for delaying the deactivation of glutaryl amidase during enzyme catalysis
AssigneeAventis Pharma Deutschland GmbH
InventorsJürgen Bongs, Johannes Meiwes, Wolfgang Kruse, Klaus-Peter Koller
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Abstract
The present invention relates to a process for delaying the deactivation of glutaryl amidase during enzyme catalysis. The enzyme catalyst is, after maximum conversion of the substrate, separated off by filtration and can be reused in a subsequent reaction batch. In each reaction cycle, the enzyme catalyst loses activity. The inactivation of the enzyme catalyst can be delayed by bringing it into contact with a thiol during the reaction.
Claims8
A process for delaying the deactivation of glutaryl amidase during catalysis by said amidase in a substrate solution, comprising bringing the glutaryl amidase into contact with at least one thiol.
A process for preparing 7-aminocephalosporic acid, comprising
- a) reacting glutaryl-7-aminocephalosporic acid with glutaryl amidase, and
- b) delaying the deactivation of said glutaryl am dase by bringing said glutaryl amidase into the contact with at least one thiol.
Dependencies are inferred from the claim text.