Composition and method for treating tissue samples
AssigneeCell Marque Corporation
InventorsNora Betyousef Aghassi, Kim Franceschini, Paul John Ardi
Life of this patent
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Abstract
Compositions and methods are described for enhancing the immunohistochemical staining of tissue samples. Compositions include a single solution, which includes at least one surfactant, adapted to remove an embedding medium of a tissue sample, rehydrate the tissue sample, and enhance the immunohistochemical staining of the tissue in relation to immunohistochemical staining of unreacted tissue. Methods include heating the tissue sample with the composition to enhance the immunohistochemical staining of the tissue.
Claims12
A composition for enhancing antigenicity of a formaldehyde-fixed, paraffin-embedded, tissue, the composition being a buffered solution comprising:
- about 0.01 moles of trisodium citrate dihydrate, about 1000 milliliters of water and about 18.5 milliliters of a mixture having by weight about 5.8% ethylene glycol monobutyl ether, about 3.75% nonylphenol ethoxylate, about 1.5% tetrapotassium pyrophosphate and about 88.95% water;
- and
- the composition having a pH between about 5.96 and about 6.04.
An aqueous composition for enhancing antigenicity of a formaldehyde-fixed, paraffin-embedded, slide-mounted tissue, the composition having a pH between about 5.96 and about 6.04 and comprising:
- greater than 0% and up to about 25% by volume of a mixture having by weight about 5.8% ethylene glycol monobutyl ether, about 3.75% nonylphenol ethoxylate, about 1.5% tetrapotassium pyrophosphate and about 88.95% water;
- and
- greater than 0% and up to 10% by weight of citric acid and alkaline citrate salts.
A method for enhancing immunochemical staining of a paraffin-embedded, formalin-fixed tissue with a single solution, the method comprising:
- immersing said tissue for a period of at least about 10 minutes and a temperature of at least 80° C. in an aqueous, paraffin-removing, tissue-activating agent solution having a pH from about 2 to about 8, said solution containing greater than 0% and up to about 10% by weight of at least one surfactant capable of, upon heating, removing paraffin from the tissue in contact therewith and also containing at least one tissue activating agent selected from a buffer or a salt;
- thereafter removing the tissue from the solution including washing the tissue to remove residual solution;
- wherein the immunochemical staining of the immersed, heated, and washed tissue is enhanced over that of formalin-fixed, paraffin-embedded tissue not so immersed and heated.
Dependencies are inferred from the claim text.