Human cancer cell line malignantly altered by expression of angiogenic factor

A method for producing an angiogenesis inhibitory composition, comprising the steps of obtaining a substance having angiogenesis inhibitory activity the method of bringing a candidate substance into contact with a cell, having angiogenesis-inducing ability, and selecting a substance having activity of inhibiting the angiogenesis-inducing ability of the cell, wherein said cell is a cell to which angiogenesis-inducing ability has been imparted by introducing a gene of an angiogenic factor selected from the group consisting of vascular endothelial growth factor, platelet-derived growth factor, hepatocyte growth factor, fibroblast growth factor, transforming growth factor-α, epithelial growth factor, interleukin 1 and interleukin 8, into a tumorigenic human or animal cancer cell having low angiogenesis-inducing ability, and selecting a cell that overexpresses the angiogenic factor, and preparing the composition from the obtained substance and a carner.

A method for screening for a substance having anticancer activity, comprising the steps of bringing a candidate substance into contact with a cell, and selecting a substance having anticancer activity on the cell, wherein said cell is a cell to which angiogenesis-inducing ability has been imparted by introducing a gene of an angiogenic factor selected from the group consisting of vascular endothelial growth factor, platelet-derived growth factor, hepatocyte growth factor, fibroblast growth factor, transforming growth factor-α, epithelial growth factor, interleukin 1 and interleukin 8, into a tumorigenic human or animal cancer cell having low angiogenesis-inducing ability, and selecting a cell that overexpresses the angiogenic factor.

A method for producing an anticancer composition, comprising the steps of obtaining a substance having anticancer activity by the method of bringing a candidate substance into contact with a cell, and selecting a substance having anticancer activity on the cell, wherein said cell is a cell to which angiogenesis-inducing ability has been imparted by introducing a gene of an angiogenic factor selected from the group consisting of vascular endothelial growth factor, platelet-derived growth factor, hepatocyte growth factor, fibroblast growth factor, transforming growth factor-α, epithelial growth factor, interleukin 1 and interleukin 8, into a tumorigenic human or animal cancer cell having low angiogenesis-inducing ability, and selecting a cell that overexpresses the angiogenic factor, and preparing the composition from the obtained substance and a carrier.