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US 6,649,362 B2Granted
Screening method for an agent having an effect on a sphingosine kinase signaling pathway
AssigneeMedvet Science Pty. Ltd.
InventorsJennifer Gamble, Mathew Vadas, Pu Xia, Phillip Barter, Kerry-Anne Rye, Brian Wattenberg, Stuart Pitson
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Abstract
A screening method for identifying a therapeutic candidate for a coronary heart disease or an inflammatory condition is disclosed. The screening method tests for the presence or absence of an effect by a putative therapeutic agent on a component of a sphingosine kinase signaling pathway.
Claims8
A screening method for identifying a therapeutic candidate for a coronary heart disease or an inflammatory condition that comprises
- (i) bringing at least one agent into contact with at least one component of a sphingosine kinase signaling pathway, under conditions such that an effect on the activity of said component is detectable, and
- (ii) detecting the presence or absence of said effect, whereby detecting said effect indicates said agent as a therapeutic candidate with respect to coronary heart disease or an inflammatory condition.
A screening method that comprises
- (i) identifying a disease condition in which up-regulation of an adhesion molecule is harmful,
- (ii) providing a solid support to which sphingosine is bound;
- (iii) contacting the solid support with sphingosine kinase and 33P-ATP in the presence or absence of an agent;
- and
- (iii) comparing an amount of bound 33P produced in the presence of said agent with that produced in the absence of said agent, whereby a difference in said amount indicates said agent as a therapeutic candidate with respect to said disease.
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Dependencies are inferred from the claim text.