Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities
AssigneeCaliper Technologies Corp.
InventorsJ. Wallace Parce, C. Nicholas Hodge, H. Garrett Wada
Life of this patent
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Abstract
Methods of monitoring transporter activity, gradient induced activity, and binding activity in microscale systems, as well as corresponding microscale devices, systems and kits are provided.
Claims19
A method of determining an effect of a compound on a transporter activity of a component, the method comprising:
- concomitanly flowing through a first channel a first component comprising the transporter activity, a transportable molecule that interacts with said first component, and a second component comprising a receptor to said transportable molecule that competitively binds with the transportable molecule;
- detecting a first signal produced by competitive binding of the receptor to the transportable molecule;
- concomitantly flowing through the first channel the first component, the transportable molecule, the second component, and a third component;
- detecting a second signal produced by the competitive binding of the receptor to the transportable molecule in the presence of the third component;
- and
- comparing the first and second signals to determine the effects of the third component on the transporter activity of the first component.
A kit comprising a first and second component, the first component comprising a transporter activity and the second component being capable of producing a signal upon exposure to a transportable molecule which is transportable by the first component, the components comprising a carrier moiety or set of carrier moieties, the carrier moiety or set of carrier moieties comprising a receptor or transporter, the carrier or carrier set comprising one or more of:
- a cell, a liposome, an organelle, a protein, and a protein-lipid complex.
Dependencies are inferred from the claim text.