Lipoxygenase proteins and nucleic acids
AssigneeVanderbilt University
InventorsAlan R. Brash, William E. Boeglin, Mitsuo Jisaka
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Abstract
Isolated and purified lipoxygenase proteins and nucleic acids are described. Particularly, a novel human 15(S) lipoxygenase (15-Lox-2) protein and cDNA and a cDNA for mouse 8S-lipoxygenase are described. Recombinant host cells, recombinant nucleic acids and recombinant proteins are also described, along with methods of producing each. Isolated and purified antibodies to 15-Lox-2 and 8-Lox, and methods of producing the same, are also described.
Claims6
An isolated and purified biologically active 15-Lox-2 polypeptide capable of converting arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, said 15-Lox-2 polypeptide containing an iron ligand comprising a serine, wherein the 15-Lox-2 polypeptide comprises an amino acid sequence at least 80% identical to the amino acid sequence of SEQ ID NO:2.
An isolated and purified biologically active 15-Lox-2 polypeptide, capable of converting arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, the 15-Lox-2 polypeptide containing an iron ligand comprising a serine, wherein the 15-Lox-2 polypeptide comprises the amino acid sequence of SEQ ID NO:2.
Dependencies are inferred from the claim text.