Solid phase synthesis
AssigneeQuiatech AB
InventorsMarek Kwiatkowski, Mats Nilsson, Ulf Landegren
Life of this patent
Estimating the term…
Abstract
The present invention relates to a support system for solid phase synthesis of oligomers, such as oligonucleotides, wherein the starting compound is bound to the support via a disiloxyl linkage. Furthermore, the invention relates to a method for synthesis of oligonucleotides on a solid support. The support system comprises a stable disiloxyl linkage providing high nucleoside loadings to the support and the method allows convenient non-laborious oligomer synthesis.
Claims27
A support system for solid phase synthesis of oligomers, said support system comprising a support and a hydroxyl-containing compound bound to the support via a disiloxyl linkage, wherein the disiloxyl group of said disiloxyl linkage is between said support and said hydroxyl-containing compound, and wherein said disiloxyl group of said disiloxyl linkage is adjacent said hydroxyl-containing compound.
A method for synthesizing an oligonucleotide, said method comprising:
- a) providing a support system comprising a hydroxyl-containing compound bound to a support via a disiloxyl linkage, wherein the disiloxyl group of said disiloxyl linkage is between said support and said hydroxyl-containing compound, and wherein said disiloxyl group of said disiloxyl linkage is adjacent said hydroxyl-containing compound;
- and
- b) condensing a 5′-protected nucleoside phosphoramidite on said support system.
A method for combinatorial chemical synthesis, said method comprising:
- a) providing a support system comprising a hydroxyl-containing compound bound to a support via a disiloxyl linkage, wherein the disiloxyl group of said disiloxyl linkage is between said support and said hydroxyl-containing compound, and wherein said disiloxyl group of said disiloxyl linkage is adjacent said hydroxyl-containing compound;
- and
- b) reacting said hydroxyl-containing compound with a non-nucleoside hydroxyl-containing compound.
A support system for solid phase synthesis of oligomers, said support system comprising a support, a linker, and a hydroxyl-containing compound bound to the support via a disiloxyl linkage, wherein the disiloxyl group of said disiloxyl linkage is between said linker and said hydroxyl-containing compound, and wherein said disiloxyl group of said disiloxyl linkage is adjacent said hydroxyl-containing compound.
Dependencies are inferred from the claim text.