Process for isolating IgG and IgA
AssigneeBaxter Aktiengesellschaft
InventorsHeinz Leibl, Regine Tomasits, Josef Mannhalter, Hermann Wolf, Martha Eibl
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Abstract
A method for the separation of IgG and IgA from an immunoglobulin-containing starting material is described, whereby the method is characterized in that (i) IgG and optionally IgA are adsorbed to a solid inorganic carrier material, (ii) IgA is isolated from the eluate, optionally after selective desorption, whereas IgG remains on the carrier material, and optionally (iii) IgG is isolated from the adsorbate. Furthermore, an IgA preparation is disclosed which demonstrates a low tendency to form aggregates.
Claims10
A method for isolating IgA from an immunoglobulin-containing starting material, comprising
- (i) contacting the immunoglobulin-containing starting material with a solid inorganic carrier material such that IgG from the immunoglobulin-containing starting material is adsorbed to the solid inorganic carrier material and an eluant and adsorbate are formed;
- (ii) isolating IgA from the eluant, wherein IgG remains on the solid inorganic carrier material, and wherein the isolated IgA has a low tendency to form aggregates, and
- (iii) isolating IgG from the adsorbate.
A method of isolating IgA from an immunoglobulin-containing starting material, comprising
- (i) contacting the immunoglobulin-containing starting material with a solid inorganic carrier material selected from the group consisting of magnesium silicates, silicic acid, diatomaceous earth and calcium phosphate, wherein during the contacting IgG from the immunoglobulin-containing starting material is adsorbed to the solid inorganic carrier material and an eluant is formed, and
- (ii) isolating IgA from the eluant, whereas IgG remains on the solid inorganic carrier material, and wherein the isolated IgA has a low tendency to form aggregates.
A method of isolating IgA that has a low tendency to form aggregates, comprising
- (i) contacting an immunoglobulin-containing starting material with a solid inorganic carrier material selected from the group consisting of magnesium silicates, silicic acid, diatomaceous earth and calcium phosphate, such that IgG from the immunoglobulin-containing starting material is adsorbed to the solid inorganic carrier material and an eluant is formed, wherein IgA is not bound to the solid inorganic carrier material, and
- (ii) isolating IgA from the eluant.
Dependencies are inferred from the claim text.