Method for remediation of an environment contaminated with carbon tetrachloride
AssigneeBoard of Trustees of Michigan State University
InventorsCraig Criddle, Michael Dybas, Greg Tatara
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Abstract
A method of remediating an environment contaminated with carbon tetrachloride by introducing Pseudomonas strain sp. KC (PsKC) into the environment under iron limiting conditions and converting the carbon tetrachloride directly to carbon dioxide and a non-volatile water soluble fraction. Further, conditions providing a niche advantage for PsKC in accordance with the invention allow for the use of PsKC as a vector in various other environmental conditions for introducing specific activities of the PsKC, whether naturally occurring or genetically altered, into those environments.
Claims5
A method of remediating an environment of soil or water in situ containing diverse microbial populations and contaminated with carbon tetrachloride which comprises:
- (a) first adjusting the environment to a pH of 7.8 to 9.2;
- and
- (b) then introducing a culture of Pseudomonas strain sp. KC (PsKC) deposited as DSM 7136 and ATCC 55595 into the environment and under anaerobic conditions, in a number and at a temperature sufficient for the PsKC to convert the carbon tetrachloride directly to carbon dioxide and a nonvolatile water soluble fraction, wherein the PsKC converts the carbon tetrachloride at the pH without producing chloroform and wherein the PsKC has been grown in a culture medium to produce the culture and then introduced into the environment containing the diverse microbial populations.
Dependencies are inferred from the claim text.