DSP-5 dual-specificity phosphatase
AssigneeCEPTYR, Inc.
InventorsRalf M. Luche, Bo Wei
Life of this patent
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Abstract
Compositions and methods are provided for the treatment of conditions associated with cell proliferation, cell differentiation and cell survival. In particular, the dual-specificity phosphatase DSP-5, and polypeptide variants thereof that stimulate dephosphorylation of DSP-5 substrates, are provided. The polypeptides may be used, for example, to identify antibodies and other agents that inhibit DSP-5 activity. The polypeptides and agents may be used to modulate cell proliferation, differentiation and survival.
Claims17
An isolated polynucleotide that encodes a dual specificity phosphatase-5 (DSP-5) substrate trapping mutant polypeptide in which a DSP-5 polypeptide comprising a sequence set forth in SEQ ID NO:3 has a substitution of an amino acid residue selected from the group consisting of (i) the aspartic acid residue at position 84 of SEQ ID NO:3 and (ii) the cysteine residue at position 115 of SEQ ID NO:3, such that the DSP-5 substrate trapping mutant polypeptide retains the ability to bind a DSP-5 substrate, and such that the ability of the DSP-5 substrate trapping mutant polypeptide to dephosphorylate the DSP-5 substrate is reduced relative to the DSP-5 polypeptide.
An isolated polynucleotide that encodes a dual specificity phosphatase-5 (DSP-5) substrate trappiny mutant polypeptide in which a DSP-5 polypeptide comprising the sequence set forth in SEQ ID NO:3, differs in one or more deletions, additions, insertions, or substitutions at no more than 25% of the residues in SEQ ID NO:3, and in which the DSP-5 polypeptide comprising the sequence set forth in SEQ ID NO:3 has a substitution of an amino acid residue selected from the group consisting of (i) the aspartic acid residue at position 84 of SEQ ID NO:3 and (ii) the cysteine residue at position 115 of SEQ ID NO:3, such that the DSP-5 substrate trapping mutant polypeptide retains the ability to bind a DSP-5 substrate, and such that the ability to dephosphorylate the DSP-5 substrate is reduced relative to the DSP-5 polypeptide.
Dependencies are inferred from the claim text.