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US 6,645,749 B2Granted
Lipolytic enzyme
AssigneeNovozymes A/S
InventorJesper Vind
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Abstract
The present invention is directed to phospholipase from Fusarium solani (MUCL38667) and analogues thereof, nucleic acid constructs, recombinant expression vectors, and recombinant host comprising the nucleic acid sequences encoding the phospholipase from Fusarium solani (MUCL38667) and analogues thereof, methods of making same, and method of using the phosopholipases of the present invention in baking methods, baking compositions and detergent compositions.
Claims9
A nucleic acid sequence which comprises:
- a) the DNA sequence encoding a mature lipolytic enzyme cloned into a plasmid present in Eseherichia coli DSM 14361,
- b) the DNA sequence encoding a mature lipolytic enzyme shown in SEQ ID NO:
- 2,
- c) an analogue of the sequence defined in a) or b) which encodes a lipolytic enzyme and
- i) has at least 95% identity with said DNA sequence, or
- ii) hybridizes with a complementary strand of said a) or SEQ ID NO:1 under hybridization conditions comprising prehybridizing in a solution of 5×SSC, 5×Denhardt's solution, 0.5% SDS and 100 μg/ml of denatured sonicated salmon sperm DNA, followed by hybridization in the same solution for 12 hours at approx. 45° C., followed by washing in 2×SSC, 0.5% SDS for 30 minutes at a temperature of at least 70° C., or
- d) a complementary strand of a), b) or c).
4 more elements
Dependencies are inferred from the claim text.